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Nematocida parisii


Product category
Product type
Parasitic protozoan
Strain designation
Type strain
Isolation source
Wild-caught Caenorhabditis elegans isolated from compost pit
Geographical isolation
France; Ile-de-France; Franconville
Product format
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Reference strain for genome sequencing project

Handling information

In vivo cultivation, Caenorhabditis elegans
Handling procedure

Storage and Culture Initiation

Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week.

Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature may result in death of the culture.

  1. Thaw a frozen ampule at room temperature. Immediately after thawing, transfer contents to a lawn of Escherichia coli OP50-1 (Caenorhabditis Genetics Center, University of Minnesota) grown on a 6-cm NGM plate. Add synchronized C. elegans (L1s or L4/young adults) to the plate and incubate for 2 days at 25oC.
  2. Remove worms from the NGM plate and examine by DIC microscopy at 630X for the presence of meronts or spores.
    NOTE: A minimum of 20 worms should be examined for evidence of infection.
Culture maintenance
  1. Place 1-3 infected donor adult worms on a lawn of Escherichia coli OP50-1 grown on a 6-cm NGM plate.
  2. Add 200–300 L1 recipient worms to the plate and co-incubate for 2 days at 25°C.
  3. Remove worms and examine by DIC microscopy at 630X for the presence of meronts or spores.
Reagents for cryopreservation

M9 buffer
KH2PO4, 3.0 g
Na2HPO4, 6.0 g
NaCl, 5.0 g
MgSO4 (1M), 1.0 ml
Distilled H2O, 1.0 L
Dissolve ingredients in 1 L of distilled water. Distribute 200 to 500 ml aliquots into appropriate sized bottles and autoclave for 15 minutes.

  1. To harvest the Nematocida culture, add 5 ml of M9 buffer to an infected NGM plate and transfer the suspension to a 15 ml centrifuge tube.
  2. Centrifuge at 200 x g for 5 min. Remove the supernatant, resuspend the pellet in 5 ml of M9 buffer, and repeat the centrifugation step.
  3. Repeat step 2 a minimum of five times in order to wash the infected worms.
  4. After the last wash, resupend the pellet in 1 ml of M9 buffer and transfer the suspension to a 2 ml microcentrifuge tube. Add Silicon carbide beads (BioSpec Products, Inc.) to the tube and vortex for 1 minute. Repeat the procedure 4-5 times.  Filter the worm extract through a Whatman filter paper number 1 to remove eggs and any remaining intact worms.
  5. Perform a spore count of the worm extract and adjust the concentration to ≥ 3 x 107 spores/ml.
    NOTE: If the concentration of spores is too low, harvest infected worms from additional NGM plates to yield the desired concentration.
  6. Mix the extract with an equal volume of M9 buffer containing 30% glycerol. The final concentration of the extract will be ≥ 1.5 x 107 spores/ml and 15% glycerol.
  7. Dispense 70 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryovials.
  8. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1o C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
  9. Store frozen ampules in either the vapor or liquid phase of a nitrogen refrigerator.


ER Troemel

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Troemel ER, et al. Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegans. PLoS Biol. 6: 2736-2752, 2008. PubMed: 19071962

Nematocida parisii ERTm1 genome sequencing project Available from: NCBI BioProject

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