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Komagataella phaffii Kurtzman

MYA-5012

This product is an ATCC manufactured and accessioned progeny of 20864 cited in US Pat. No. 5,102,789.
Product category
Fungi
Product type
Yeast
Strain designation
GS115 [SMD 83]
Type strain
No
Genome sequenced strain
Yes
Former name
Pichia pastoris (Guilliermond) Phaff
Isolation source
Mutant derived from NRRL Y-11430
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Transformation host
Transformation host for expression of beta-galactosidase
Transformation host for expression of epidermal growth factor
Transformation host for expression of alcohol oxidase
Transformation host for expression of recombinant Bordetella pertussis pertactin
Transformation host for expression of Bacillus stearothermophilus alanine carboxypeptidase gene, dacA
Transformation host for expression of HIV-1 envelope protein
Transformation host for expression of Geotrichum candidum lipase A and B
Transformation host for expression of recombinant human serum albumin
Transformation host for expression of recombinant human angiostatin
Transformation host for expression of synthetic tick anticoagulant peptide gene
Transformation host for expression of bovine enterokinase
Transformation host for expression of coffee bean alpha-galactosidase
Preceptrol
No

Characteristics

Ploidy
Haploid
Genotype
AOX1 his4-
Comments
Protein secretory pathway
Screening for multiple copy gene inserts
Komagataella pastoris was once used as a synonym for Pichia pastoris.
The sequences from the D1D2 region of LSU rDNA identified this strain as Komagataella phaffii.
Genome sequencing strain (Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, UGent, Belgium).

Handling information

Medium
Temperature
20-25°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
  1. To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.
  2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
  3. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.

Quality control specifications

Sequenced data
D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGTCCACCTTTGAAGTATTGACGTGGTTGGGAGAGGCAATGTGCACAAGTCGCCTGGAACGGCCGCCAAAGAGGGTGATAGCCCCGTAGCACGGTCAACCTTCCCAGCCGACAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAAGGTGGTAGGGTCCATCAAAGACTAAATATCAGCGAGAGACCGATAGCAAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAACAGTGCGTGAAATTGGGGGGAGGGAAGGCAAGGCCACGACAAGTGGTCCTGCGC

Verification method
Whole-genome Sequencing

History

Deposited as
ATCC accessioned progeny of Komagataella phaffii strain GS115 [SMD 83] cited in US Pat. No. 5,102,789 as 20864.
Cross references
GenBank KU729167 D1/D2 region of 26S rRNA gene

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Buckholz RG. Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same. US Patent 5,268,273 dated Dec 7 1993

Vozza LA, et al. Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Bio-Technology 14: 77-81, 1996. PubMed: 9636316

Payne WE, et al. An inducible acid phosphatase from the yeast Pichia pastoris: characterization of the gene and its product. Gene 163: 19-26, 1995. PubMed: 7557473

Zhu A, et al. High-level expression and purification of coffee bean alpha- galactosidase produced in the yeast Pichia pastoris. Arch. Biochem. Biophys. 324: 65-70, 1995. PubMed: 7503561

Cregg JM, et al. Pichia pastoris as a host system for transformations. Mol. Cell. Biol. 5: 3376-3385, 1985. PubMed: 3915774

View All Curated Citations for this Product

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