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3 Strain Tagged Genomic DNA Even Mix

MSA-1014

This NGS Standard is a mock microbial community that can be used as a spike-in control for 16S rRNA and shotgun metagenomic sequencing assays. This product comprises genomic DNA prepared from three fully characterized and authenticated ATCC Genuine Cultures that contain unique synthetic 16S tags incorporated into the bacterial genome. Each tag consists of four artificial variable regions (corresponding to V1 through V4 of 16S rRNA gene) flanked by conserved regions for PCR amplification.

Total cells: 6.0 x 107 genome copies/vial ± 1 log*
*Whole cell concentrations indicate ATCC manufacturing specifications and are provided as a reference only
Product category
Bacteria
Product type
Nucleic acid
NGS standard
Components
Nucleic acid extraction from:
33.3% Escherichia coli with Tag 1
33.3% Clostridium perfringens with Tag 2
33.3% Staphylococcus aureus with Tag 3
Applications
Bioinformatics
Microbiome research
Next-generation sequencing
Product format
Frozen
Shipping information
Stored in 10 mM Tris-HCL pH 8.5
Storage conditions
-20°C or colder
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Price: $411.00 ea
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications

NGS Standards enable the optimization of metagenomics workflows and research applications, providing reliable comparative data while improving assay consistency.

This product is ideal for use as a spike-in control in assay development, library preparation, quality control, and data normalization for both 16S rRNA and shotgun metagenomics approaches.

Characteristics

Comments
This NGS Standard is a mock microbial community that can be used as a spike-in control for 16S rRNA and shotgun metagenomic sequencing assays. This product comprises genomic DNA prepared from three fully characterized and authenticated ATCC Genuine Cultures that contain unique synthetic 16S tags incorporated into the bacterial genome. Each tag consists of four artificial variable regions (corresponding to V1 through V4 of 16S rRNA gene) flanked by conserved regions for PCR amplification.

Total cells: 6.0 x 107 genome copies/vial ± 1 log*
*Whole cell concentrations indicate ATCC manufacturing specifications and are provided as a reference only

Handling information

Handling notes
Aliquoting is highly recommended to avoid multiple freeze­-thaws, which can damage the DNA.

Quality control specifications

Volume
55 µL

History

Special collection
NGS Standards

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

For product-related inquiries and issues, contact Technical Service:

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