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pMCV1-E-D+I purified plasmid DNA

MBA-371

This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The insert is 25.487 kb and contains an internal EcoRI site. Sequence of the insert corresponds to nucleotides 83880-109367 of Genbank sequence U60315 for the complete genome of the virus. The internal EcoRI site is at nt 107692.
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Purified plasmid DNA
Storage conditions
2°C to 8°C
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Detailed product information

Characteristics

Comments
This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid. The insert is 25.487 kb and contains an internal EcoRI site. Sequence of the insert corresponds to nucleotides 83880-109367 of Genbank sequence U60315 for the complete genome of the virus. The internal EcoRI site is at nt 107692.

Vector information

Construct size (kb)
29.732
Intact vector size
4.245
Vector name
pACYC184
Type of vector
cloning
Vector information
Insert site: EcoRI
Primer yE-1 (forward): 5’-GTCGTGGTATTCACTCCAGAGCGAT-3’
Primer yE-2 (reverse): 5’-GCATTTCAGTCAGTTGCTCAATGTACCTATA-3’
Markers
tetR

Insert information

Insert size (kb)
25.487
Insert information
Insert ends: EcoRI
Source: Molluscum contagiosum virus (MCV)
Genbank accession: U60315, partial
Nucleotides 1-25487 of the insert correspond to nucleotides 83880-109367 of Genbank U60315
Insert end
EcoRI

Handling information

Handling procedure
Plasmid can be transformed into a suitable Escherichia coli host using standard protocols and then grown on LB + 10-20 µg/mL of tetracycline at 37°C.
Handling notes
Restriction digests of the clone gave the following sizes (in kb): EcoRI – 4.25, 1.7, 23.8.
–ATCC Staff

History

Depositors
J Bugert
Cross references
GenBank U60315 Molluscum contagiosum virus subtype 1, complete genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497

Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026

Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425

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