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pTR-UF-11 plasmid in E. coli

MBA-331

SmaI cuts in both AAV ITRs (these can be unstable in bacteria). When culturing the bacteria containing AAV ITRs, do not culture for more than 16-18 hours at 37°C because deletions may occur especially in stationary cultures. A missing ITR can be tolerated in about 20% of a plasmid prep. SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning. If an ITR is deleted, then the SmaI digests will generate larger molecular weight bands.
Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Storage conditions
-80°C or colder
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact.

Characteristics

Comments
SmaI cuts in both AAV ITRs (these can be unstable in bacteria). When culturing the bacteria containing AAV ITRs, do not culture for more than 16-18 hours at 37°C because deletions may occur especially in stationary cultures. A missing ITR can be tolerated in about 20% of a plasmid prep. SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning. If an ITR is deleted, then the SmaI digests will generate larger molecular weight bands.
Mycoplasma contamination
Not detected

Vector information

Markers
ampR

Handling information

Host
Escherichia coli SURE Cells from Stratagene
Medium
Temperature
37°C
Handling notes
Restriction digests on the vector gave the following results (in Kb): PstI- 2.8, 1.9, 1.2, 1.0, 0.2, SmaI- 4.2, 3.0, faint band at 7.2 (indicates deletion of one ITR), NotI- 6.5, 0.7
--ATCC staff

Quality control specifications

Sequenced data

1 GGGGGGGGGG GGGGGGGGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC 61 GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA 121 GCGCGCAGAG AGGGAGTGGC CAACTCCATC ACTAGGGGTT CCTAGATCTG AATTCGGTAC 181 CCTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC 241 CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA 301 TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT 361 CAATGGGTGG ACTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG 421 CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG 481 TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT 541 ACCATGGTCG AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA 601 CCCCCAATTT TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG 661 GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG CGGGGCGAGG GGCGGGGCGG GGCGAGGCGG 721 AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC GCGCTCCGAA AGTTTCCTTT TATGGCGAGG 781 CGGCGGCGGC GGCGGCCCTA TAAAAAGCGA AGCGCGCGGC GGGCGGGAGT CGCTGCGACG 841 CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT 901 GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA 961 GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT 1021 CCGGGAGGGC CCTTTGTGCG GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG TGTGTGTGCG 1081 TGGGGAGCGC CGCGTGCGGC CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC 1141 GGGGCTTTGT GCGCTCCGCA GTGTGCGCGA GGGGAGCGCG GCCGGGGGCG GTGCCCCGCG 1201 GTGCGGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG TGGGGGGGTG 1261 AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC CCTCCCCGAG 1321 TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTAC GGGGCGTGGC GCGGGGCTCG 1381 CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG CCGCCTCGGG 1441 CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCCGGAG CGCCGGCGGC TGTCGAGGCG 1501 CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG GGACTTCCTT 1561 TGTCCCAAAT CTGTGCGGAG CCGAAATCTG GGAGGCGCCG CCGCACCCCC TCTAGCGGGC 1621 GCGGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC CTTCGTGCGT 1681 CGCCGCGCCG CCGTCCCCTT CTCCCTCTCC AGCCTCGGGG CTGTCCGCGG GGGGACGGCT 1741 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA 1801 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 1861 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCCT CGAAGATCTA GGCCTGCAGG 1921 CGGCCGCCGC CACCATGAGC AAGGGCGAGG AACTGTTCAC TGGCGTGGTC CCAATTCTCG 1981 TGGAACTGGA TGGCGATGTG AATGGGCACA AATTTTCTGT CAGCGGAGAG GGTGAAGGTG 2041 ATGCCACATA CGGAAAGCTC ACCCTGAAAT TCATCTGCAC CACTGGAAAG CTCCCTGTGC 2101 CATGGCCAAC ACTGGTCACT ACCCTGACCT ATGGCGTGCA GTGCTTTTCC AGATACCCAG 2161 ACCATATGAA GCAGCATGAC TTTTTCAAGA GCGCCATGCC CGAGGGCTAT GTGCAGGAGA 2221 GAACCATCTT TTTCAAAGAT GACGGGAACT ACAAGACCCG CGCTGAAGTC AAGTTCGAAG 2281 GTGACACCCT GGTGAATAGA ATCGAGCTGA AGGGCATTGA CTTTAAGGAG GATGGAAACA 2341 TTCTCGGCCA CAAGCTGGAA TACAACTATA ACTCCCACAA TGTGTACATC ATGGCCGACA 2401 AGCAAAAGAA TGGCATCAAG GTCAACTTCA AGATCAGACA CAACATTGAG GATGGATCCG 2461 TGCAGCTGGC CGACCATTAT CAACAGAACA CTCCAATCGG CGACGGCCCT GTGCTCCTCC 2521 CAGACAACCA TTACCTGTCC ACCCAGTCTG CCCTGTCTAA AGATCCCAAC GAAAAGAGAG 2581 ACCACATGGT CCTGCTGGAG TTTGTGACCG CTGCTGGGAT CACACATGGC ATGGACGAGC 2641 TGTACAAGTG AGCGGCCGCG GGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA 2701 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC 2761 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT 2821 TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAG TCGACCTCGA GCAGTGTGGT 2881 TTTGCAAGAG GAAGCAAAAA GCCTCTCCAC CCAGGCCTGG AATGTTTCCA CCCAAGTCGA 2941 AGGCAGTGTG GTTTTGCAAG AGGAAGCAAA AAGCCTCTCC ACCCAGGCCT GGAATGTTTC 3001 CACCCAATGT CGAGCAACCC CGCCCAGCGT CTTGTCATTG GCGAATTCGA ACACGCAGAT 3061 GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT AAGGTGACGC GTGTGGCCTC 3121 GAACACCGAG CGACCCTGCA GCCAATATGG GATCGGCCAT TGAACAAGAT GGATTGCACG 3181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 3241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 3301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 3361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 3421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 3481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 3541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 3601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 3661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 3721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 3781 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 3841 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 3901 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GGGGATCCGT 3961 CGACTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT 4021 GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT 4081 AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG 4141 TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGAGAGAT 4201 CTAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA 4261 GGCCGCCCGG GCAAAGCCCG GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA 4321 GCGAGCGCGC AGAGAGGGAG TGGCCAACCC CCCCCCCCCC CCCCCTGCAG CCCTGCATTA 4381 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 4441 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 4501 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 4561 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 4621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 4681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 4741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 4801 TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 4861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 4921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 4981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 5041 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 5101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 5161 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 5221 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 5281 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 5341 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 5401 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 5461 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 5521 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 5581 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 5641 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 5701 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 5761 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 5821 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 5881 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 5941 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 6001 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 6061 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 6121 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 6181 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 6241 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 6301 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 6361 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC 6421 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 6481 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 6541 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 6601 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 6661 CAGGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC 6721 TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC 6781 GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC 6841 TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA 6901 CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG 6961 AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG 7021 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC 7081 ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CGCGCCATTC GCCATTCAGG 7141 CTACGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGGCTGCA

History

Depositors
University of Florida

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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Burger C. et al. Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system. Molecular Therapy : 10(2):302-17, 2004 PubMed: 15294177

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