pNIAysic-7 plasmid in E. coli TOP10

1. Transfer to growth media by inoculating a loopful from the thawed cryovial to a test tube containing 5 mL LB+50 µg/mL of ampicillin broth. 
2. A loopful of culture can also be streaked on an LB + amp agar plate. Incubate cultures at 37°C. 
3. Isolate DNA using standard plasmid preparation procedures.

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