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pGRN145 plasmid in E. coli GC10


pGRN145 is an expression plasmid containing the human telomerase reverse transcriptase (hTERT) cDNA in E. coli GC10.
Molecular biology
Product format
Shipping information
Escherichia coli GC10 containing the plasmid in glycerol stock
Storage conditions
-80°C or colder


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
Expression vector [telomerase reverse transcriptase] (EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit)


Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
Vector information
hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
Ori: 12165-12747
Restriction digests of this clone gave the followi

Insert information

Insert size (kb)
Insert information
Gene: Telomerase reverse transcriptase (TERT)
Source: Human
GenBank accession: NM_003219

Handling information

Handling procedure

Streak a loopful of culture on an LB + 37 µg/mL chloramphenicol agar plate. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures.



Geron Corporation
Cross references
GenBank NM_003219

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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