Mouse Notch1 in pcDNA1 in Escherichia coli MC1061/P3
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Asceptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well. Transfer 100 µL to a test tube containing 5 mL LB+50 µg/mL of ampicillin. A loopful of culture can also be streaked on an LB + amp agar plate. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures.
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