Pleomorphomonas oryzae Xie and Yokota
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1. Open vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 48 hours.
Colonies on #3 agar are tiny, circular, smooth, raised, entire, and transparent.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
1. Xie CH and Yokota A. Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa. Int. J. Syst. Evol. Microbiol. 55: 1233-1237, 2005.
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rice plant, Oryza sativa
Cheng-hui Xie, personal communication