Vibrio shilonii Kushmaro et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 slant, and/or plate
5. Incubate the tubes and plate at the recommended temperature for 48 hours.
6. After growth has been achieved, additional transfers may be made or cells prepared for storage. Many Vibrio species will die off rapidly once the stationary phase is reached, even at refrigerator temperatures. To avoid frequent transfers, it is recommended that cells be frozen with a suitable cryoprotectant.
Colonies are small, entire, smooth, glistening and translucent. Cells are short slightly curved rods.
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Kushmaro A, et al. Bacterial infection and coral bleaching. Nature 380: 396, 1996.
Kushmaro A, et al. Vibrio shiloi sp. nov., the causative agent of bleaching of the coral Oculina patagonica. Int. J. Syst. Evol. Microbiol. 51: 1383-1388, 2001. PubMed: 11491336
bleached coral, Oculina patagonica, from the Mediterranean coast of Israel