Oceanimonas smirnovii Ivanova et al.
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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 24-48 hours.
Colonies on #2 agar appear circular, smooth, raised, entire, and translucent.
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seawater, Black Sea
Elena Ivanova, personal communication
Ivanova EP, et al. Oceanimonas smirnovii sp. nov., a novel organism isolated from the Black Sea. Syst. Appl. Microbiol. 28(2): 131-136, 2005. PubMed: 15830805