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2. Using a single tube of #2 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #2 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 48 hours.
Colonies on #2 agar are circular, smooth, raised, entire, and translucent.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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subseafloor alkaline serpentine mud from a serpentine mud vulcano, South Chamorro Seamount, Mariana Forearc, Pacific Ocean
Ken Takai, personal communication
Takai K, et al. Marinobacter alkaliphilus sp. nov., a novel alkaliphilic bacterium isolated from subseafloor alkaline serpentine mud from Ocean Drilling Program Site 1200 at South Chamorro Seamount, Mariana Forearc. Extremophiles. 9(1): 17-27, 2005. PubMed: 15322951