Bacillus psychrodurans Abd El-Rahman et al.
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2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or a plate.
5. Incubate the tubes and plate at 26°C for 48 to 72 hours.
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Abd El-Rahman HA, et al. Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. Int. J. Syst. Evol. Microbiol. 52: 2127-2133, 2002. PubMed: 12508879
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