Paralactobacillus selangorensis Leisner et al.
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2. From a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Use 0.1 ml of this suspension to inoculate #416 slants and 0.1ml to inoculate #416 plates.
4. Incubate tubes and plates at 30oC, under aerobic conditions, for 48 hours.
5. After 48 hours of incubation, wash cells from the slant and transfer this broth to a new slant and plate. Incubate another 48 hours under aerobic conditions. This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.
Cells are Gram positive, nonmotile rods, occurring individually and paired.
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Leisner JJ, et al. Description of Paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a Malaysian food ingredient. Int. J. Syst. Evol. Microbiol. 50: 19-24, 2000. PubMed: 10826783