Pandoraea pnomenusa Coenye et al.
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2. From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or a plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Coenye T, et al. Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov.. Int. J. Syst. Evol. Microbiol. 50: 887-899, 2000. PubMed: 10758901