Sinorhizobium americanum Toledo et al.
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2. From a single tube of #111 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate the tubes and plate at 30°C for 48-72 hours.
Cells in #111 broth are rods.
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Toledo I, et al. Sinorhizobium americanus sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico [published erratum appears in Syst. Appl. Microbiol. 26: 319, 2003]. Syst. Appl. Microbiol. 26: 54-64, 2003. PubMed: 12747410
. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 54: 1909-1910, 2004. PubMed: 15545406