Microvirga subterranea Kanso and Patel
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2. Using a single tube of #2275 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Inoculate additional tubes of broth and slants. Plates may also be inoculated to test for purity.
4. Incubate broth tube at the recommended temperature for 48 hours.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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