Atopobacter phocae Lawson et al.
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24 to 48 hours.
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Lawson PA, et al. Atopobacter phocae gen. nov., sp. nov., a novel bacterium isolated from common seals. Int. J. Syst. Evol. Microbiol. 50: 1755-1760, 2000. PubMed: 11034483