Mycobacterium montefiorense Levi et al.
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2. Using a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #173 or #90 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 8 weeks or until growth is observed
6. After growth has been achieved, additional transfers may be made.
Two colony types observed on #173 agar plates are a) large, irregular, erose, rough, opaque, and flat; and b) entire, circular, smooth, translucent, and pinpoint.
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. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 53: 1701-1702, 2003. PubMed: 14657096
lesions on captive green moray eels, Gymnothorax funebris, with granulomatous dermatitis
Levi MH, et al. Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex. J. Clin. Microbiol. 41: 2147-2152, 2003. PubMed: 12734264