Ensifer kostiensis (Nick et al.) Young
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2. From a single tube of #111 broth (5 to 6 ml), withdraw approximately 0.5 to 1 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #111 agar slant and/or plate.
5. Incubate all tubes and plate at 26°C for 48-72 hours.
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Nick G, et al. Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya. Int. J. Syst. Bacteriol. 49: 1359-1368, 1999. PubMed: 10555313
Young JM. The genus name Ensifer Casida 1982 takes priority over Sinorhizobium Chen et al. 1988, and Sinorhizobium morelense Wang et al. 2002 is a later synonym of Ensifer adhaerens Casida 1982. Is the combination "Sinorhizobium adhaerens" (Casida 1982) Willems et al. 2003 legitimate? Request for an Opinion. Int. J. Syst. Evol. Microbiol. 53: 2107-2110, 2003. PubMed: 14657154