• Quick Order
  • Careers
  • Support

Escherichia coli GFP


Download Genome Learn about the ATCC Genome Portal
Product category
Product type
Drug-resistant bacterium
Reporter-labeled bacterium
Strain designation
Type strain
Genome sequenced strain
Isolation source
Laboratory engineered
Assay development
Enteric disease research
Food testing
High-throughput screening
Infectious disease research
Product format
Storage conditions
-80°C or colder
Buy Now
Price: $620.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Food testing
Enteric research
Fluorescence detection


Multilocus Sequence Type (MLST): ST-655
Toxin genes
eae (Intimin) positive
stx1 (Shiga toxin 1) negative
stx2 (Shiga toxin 2) positive
Susceptibility profile
This clone was derived from ATCC® BAA-2219™ and contains a multi-copy vector encoding a synthetic non-Aequorea fluorescent protein. This gene is expressed under the control of the P_lac promoter and exhibits ampicillin resistance. This strain was confirmed by PCR to carry the stx2 and eaeA genes (stx1-, stx2+, eaeA+) and is toxigenic. 

Ampicillin resistance gene encoded on a plasmid, which confers resistance to 250µg/ml

Handling information

Handling procedure
  1. Open thawed vial according to enclosed instructions or visit for instructions.
  2. Aseptically transfer the entire contents to a 5­6 mL tube of #2855 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Incubate while shaking at 37°C for 16 hours.
  4. Although this item is ideally grown in broth, it will also grow on #2855 agar.
Handling notes
This strain contains a multi-copy vector encoding a synthetic non-Aequorea fluorescent protein. This protein is green in color and can be visualized using flow cytometry and imaging (Excitation: 505 nm; Emission: 525 nm) or a UV box (310 nm). Longer incubations (24-48h) may be required in order to visualize fluorescence.
A concentration of 250 µg/mL ampicillin should be maintained at all times during culture. Absence or low concentrations of ampicillin will result in plasmid loss.
This strain was confirmed by PCR to carry the stx2 and eaeA genes (stx1-, stx2+, eaeA+)
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Escherichia coli
Cross references
GenBank AYRW00000000 Escherichia coli ATCC BAA-2219, whole genome shotgun sequencing project

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.


US and Puerto Rico

Outside the US

Hours of Operation

9:00am - 5:00pm
US Eastern Time