Herminiimonas glaciei Loveland-Curtze et al. 2009
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2. Transfer thawed cells into a single tube of #18 broth (5 to 6 ml).
3. Use several drops of the suspension to inoculate additional tubes of broth, agar slants, and/or plate.
4. Incubate the tubes and plate at 30°C for 48-72 hours.
2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 48-72 hours.
This strain exhibits two different colony sizes on #18 plates.
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Miteva VI, Brenchley JE. Detection and isolation of ultrasmall microorganisms from a 120,000-year-old Greenland glacier ice core. Appl. Environ. Microbiol. 71(12): 7806-7818, 2005. PubMed: 16332755
Loveland-Curtze J, Miteva VI, Brenchley JE. Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice. Int. J. Syst. Evol. Microbiol. 59(Pt 6): 1272-1277, 2009. PubMed: 19502300