Leucobacter chromiireducens subsp. solipictus
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2. Using a single tube of #1065 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a # 1065 agar slant and/or plate.
5. Incubate the tubes and plate at 20-25oC for 48 hours.
Colonies on #1065 agar are small, entire, raised, shiny, round, moist, and glistening. Produce This strain produces yellow pigment when growth grown in under light.
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Muir RE, Tan MW. Virulence of Leucobacter chromiireducens subsp. solipictus to Caenorhabditis elegans: characterization of a novel host-pathogen interaction. Appl. Environ. Microbiol. 74(13): 4185-4198, 2008. PubMed: 18487405
Muir RE, Tan MW. Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens. Int. J. Syst. Evol. Microbiol. 57(Pt 12): 2770-2776, 2007. PubMed: 18048723