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Arthrobacter sulfonivorans Borodina et al.


Product category
Strain designation
ALL [DSM 14002]
Type strain
Isolation source
Soil from root balls of Allium aflatuense
Product format
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Degrades dimethylsulfone

Handling information

Handling procedure
1. Open vial according to enclosed instructions.

2. From a single tube of #3 or #396 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.

3. Use 0.1 ml of this suspension to inoculate #3 or #396 slants and 0.1ml to inoculate #3 or #396 plates.

4. Incubate tubes and plates at 26oC, under aerobic conditions for 3 days.

5. After 3 days of incubation, wash cells from the slant and transfer this broth to a new slant and plate.  Incubate another 3 days under aerobic conditions.  This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.

Handling notes
Growth on agar yields convex colonies, 1-2 mm in diameter, with entire margins and an opaque hue.

          Cells are Gram positive, nonmotile rods, exhibiting a rod-coccus life cycle with age.

          An alternative medium suggested by the depositor for methylotrophic growth is as follows:

Na2HPO4.2H2O, 7.9 g

KH2PO4, 1.5 g

NH4Cl, 0.8 g

MgSO4.7H2O, 0.1 g

Trace metal sol'n (see below), 10 ml

Agar (if desired), 15.0 g

Dimethylsulfone, 1.9 g

Distilled water, 1 L

Initial pH 7.3-7.4.

[Also grows on methanol, methylamine, glucose, fructose]

          Trace metal stock solution:

1.       Dissolve 50 g EDTA (disodium salt) in 400 ml water.  Dissolve 9 g NaOH in the EDTA sol'n.

2.       Dissolve the following salts individually in 30-40 ml aliquots of water and add to the EDTA-NaOH sol'n (plus 5-10 ml washings).

ZnSO4.7H2O, 1.0 g

CaCl2, 5.0 g

MnCl2.4H2O, 2.5 g

CoCl2.6H2O, 0.5 g

Ammonium molybdate, 0.5 g

FeSO4.7H2             O, 5.0 g

                CuSO4.5H2O, 0.2 g

Adjust pH to 6.0 with 1M NaOH (~ 24 ml).  Store in an amber bottle.


Deposited as
Arthrobacter sulfonivorans Borodina et al.
AP Wood
Type of isolate
Year of origin
Special collection
NSF - Bacteriology
Cross references
GenBank AF235091 16S rRNA sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Borodina E, et al. Dimethylsulfone as a growth substrate for novel methylotrophic species of Hyphomicrobium and Arthrobacter. Arch. Microbiol. 173: 425-437, 2000. PubMed: 10896224

. Validation of publication of new names and new combinations previously effectively published outside the IJSEM.. Int. J. Syst. Evol. Microbiol. 52: 685-690, 2002. PubMed: 12054225

Borodina E, et al. Enzymes of dimethylsulfone metabolism and the phylogenetic characterization of the facultative methylotrophs Arthrobacter sulfonivorans sp. nov., Arthrobacter methylotrophus sp. nov., and Hyphomicrobium sulfonivorans sp. nov.. Arch. Microbiol. 177: 173-183, 2002. PubMed: 11807567

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