Leptospira fainei Perolat et al.
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2. Incubate test tubes with screw caps lightly tightened along with the blood plate at 30oC under aerobic conditions.
3. Initial growth may take up to 14 days. Observe growth as a thin, tight band forming just below the surface of the medium. The band thickens as incubation continues. Cellular morphology under phase contrast shows active, spiral cells with flexing motility.
4. The aerobic blood plate should show no signs of growth.
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Perolat P, et al. Leptospira fainei sp. nov., isolated from pigs in Australia. Int. J. Syst. Bacteriol. 48: 851-858, 1998. PubMed: 9734039
Morey RE, et al. Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. J. Clin. Microbiol. 44(10): 3510-3516, 2006. PubMed: 17021075