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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 24 hours.
Colonies on #18 agar appear dry, raised, undulate, adhere to agar, and are translucent.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Hatayama K, et al. Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile.. Int. J. Syst. Evol. Microbiol. 55: 1539-1544, 2005.