Human Coxsackievirus B 2 (ATCC® VR-29)

Classification: Picornaviridae, Enterovirus, Human enterovirus B  /  Product Format: frozen

Classification Picornaviridae, Enterovirus, Human enterovirus B
Deposited As Coxsackievirus B2
Agent Human Coxsackievirus B 2
Strain Ohio-1
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
CF antigen may be prepared from cell cultures
Effect on Host
CPE, cell rounding and sloughing of monolayer.
Recommended Host
LLC-MK2 (ATCC® CCL-7.1™)
Growth Conditions

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

Incubation: TC, 2 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.

Effect on Host
CPE, cell rounding and sloughing of monolayer.
Name of Depositor JL Melnick
Source Stool from patient with summer grippe, Ohio, 1947
Year of Origin 1947
References

Melnick JL. . J. Exp. Med. 91: 185-195, 1950.

Cross References

Nucleotide (GenBank) : AJ295199 Human coxsackievirus B2 genomic RNA for partial polyprotein gene, isolate VR-29, ATCC.