Vaccinia virus (ATCC® VR-1354)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Deposited As Vaccinia virus, Orthopoxvirus
Agent Vaccinia virus
Strain WR (NIH TC-adapted)
Applications
testing disinfectants
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Genome Sequenced Strain

Vaccinia virus WR, complete genome

Comments

This strain is recommended by ATCC for use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified.

This tissue culture adapted WR strain of vaccinia is derived from ATCC VR-119 and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984)

Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as ATCC VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986).

Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).

Cell associated virus from infected HeLa cells

Effect on Host
CPE, cell rounding and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection:  Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted in Virus growth medium: EMEM to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation:  Incubate infected culture for 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance throughout.

Effect on Host
CPE, cell rounding and sloughing
Name of Depositor NS Cooper, B Moss, NIH
Special Collection ATCC
Source Multiple laboratory passages in cell culture of WR strain of vaccinia
References

Salzman NP, Sebring ED. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J. Virol. 1: 16-23, 1967. PubMed: 4248263

Mackett M, et al. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. Sci. USA 79: 7415-7419, 1982. PubMed: 6296831

Cross References

Nucleotide (GenBank) : AY243312.1 Vaccinia virus WR, complete genome

Nucleotide (GenBank) : S64006 E3L=double-stranded RNA-binding protein [vaccinia virus, WR, ATCC VR1354, Genomic, 633 nt].

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation