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pENTR_L1L2_LitR (ATCC® SB-1089)

Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L1L2_LitR
Depositors R Weiss, Massachusetts Institute of Technology
Applications A TetR homolog-based repressor for gene expression regulation
Vector Total size of construct (kb): 3.4249
Vector type: Entry vector
Markers: kanR
Insert Insert size (Kb): 0.65
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Preparation
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 20 μl of sterilized water to dissolve the DNA. Each vial contains 2-3 μg plasmid DNA.
Related Products ATCC SB-1069
ATCC SB-1032
ATCC SB-1085
ATCC SB-1086
ATCC SB-1090
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737