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pENTR_L4R1_pGPD-LitR (ATCC® SB-1069)

Permits and Restrictions

View Permits

Designations pENTR_L4R1_pGPD-LitR
Applications A TetR homolog-based synthetic promoter for gene expression regulation
Vector Total size of construct (kb): 3.430
Vector type: Entry vector
Markers: kanR
Insert Insert size (Kb): 0.72
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Related Products ATCC SB-1089
ATCC SB-1065
ATCC SB-1066
ATCC SB-1070
ATCC SB-1071
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).

This construct contains a synthetically designed repressible promoter comprising a TetR homolog LitR operator and a strong yeast GPD promoter

References

Stanton BC, et al. Genomic mining of prokaryotic repressors for orthogonal logic gates. Nat Chem Bio 10(2): 99-105, 2014. PubMed: 24316737

Ron Weiss, personal communication