Corneal Epithelial Cell Growth Kit (ATCC® PCS-700-040)

Permits and Restrictions

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Biosafety Level 1
Product Format frozen 1 kit
Storage Conditions -20°C (or -70°C for long-term storage)
Comments
Corneal Epithelial Cell Growth Kit (ATCC PCS-700-040) contains components that when added to Corneal Epithelial Cell Basal Medium (ATCC PCS-700-030) creates a complete ATCC® Primary Cell Solution&trad; culture environment for epithelial cells derived from normal human corneal. The serum-free medium formulation is designed to support normal corneal epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.

The final concentration for each component in ATCC complete corneal epithelial growth medium is as follows:
  • Apo-Transferrin: 5 µg/mL
  • Epinephrine: 1.0 µM
  • Extract P: 0.4%
  • Hydrocortisone: 100 ng/mL
  • L-Glutamine: 6 mM
  • rh Insulin: 5 µg/ml
  • CE Growth Factor: Proprietary formulation
Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight. 
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium. 
  3. Obtain one bottle of Corneal Epithelial Cell Basal Medium (485 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.
  6. Table 1. Corneal Epithelial Cell Growth Kit Components 

    Component                     

    Volume

    Final Concentration

    Apo-transferrin

    0.5 mL

    5 mg/mL

    Epinephrine

    0.5 mL

    1.0 mM

    Extract P

    2 mL

    0.4%

    Hydrocortisone Hemisuccinate

    0.5 mL

    100 ng/mL

    L-Glutamine

    15 mL

    6 mM

    rh Insulin

    0.5 mL

    5 µg/mL

    CE Growth Factor

    1 mL

    Proprietary formulation

    Antimicrobials and phenol red are not required for proliferation, but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 2.

    Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

    Component

    Volume

    Final Concentration

    Gentamicin-Amphotericin B Solution

    0.5 mL

    Gentamicin: 10 µg/mL

    Amphotericin B: 0.25 µg/mL

    Penicillin-Streptomycin-Amphotericin B Solution

    0.5 mL

    Penicillin: 10 Units/mL

    Streptomycin: 10 µg/mL

    Amphotericin B: 25 ng/mL

    Phenol Red

    0.5 mL

    33 µM


  7. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  8. Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.
Volume 1 kit
Sterility Tests
Negative for bacteria, fungi and yeast
Functional Tests Rate of proliferation and morphology.
A Certificate of analysis (CoA) is available upon request for each lot of Corneal Epithelial Cell Growth Kit.
C of A
Certificate of Analysis
Certificate of Analysis
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation