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Designations
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pTR-UF-11 plasmid in E. coli
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Vector Information
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Cloning sites: NotI sites (nt 1922 and 2654), AccI (nt 2862 and 3961). Transcription terminators: SV40 polyA, bovine growth hormone polyA. Suggested host: E. coli SURE (Stratagene)
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Applications
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SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact.
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Comments
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NOTE: this vector harbors AAV2 Inverted Terminal repeats (AAV ITRs)which can be unstable in bacteria. When culturing the bacteria containing AAV ITRs, do not culture for more than 16-18 hours at 37C because deletions may occur especially in stationary cultures. A missing ITR can be tolerated in about 20% of a plasmid prep. SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning. If an ITR is deleted, then the SmaI digests will generate larger molecular weight bands.
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