Azotobacter vinelandii Lipman

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #12 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #12 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #12 plate and/or #12 agar slant.
4. Incubate at 26°C for 24 to 48 hours.

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