pSLF102 (ATCC® 87620)

Applications: expression vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pSLF102
Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 7.4000000953674320
Vector: pSLF102 (plasmid)
Promoters: Promoter CaMV
Marker(s):ampR,ura4+
Construct size (kb): 7.400000095367432
Features: marker(s): ampR
marker(s): ura4+
operator: tet
promoter: CaMV
replicon: ars1
replicon: pMB1
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; HindIII--5.6, 1.8; SmaI--7.4.
The vector contains the constitutive CaMV promoter adjacent to the tet operator.
The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.
A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

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component of:ATCC 87685
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