pSLF101 (ATCC® 87619)

Applications: expression vector  /  Depositors: SL Forsburg

Designations pSLF101
Depositors SL Forsburg
Biosafety Level 1
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.8000001907348630
Intact vector size: 6.800
Type of vector: phagemid
Cloning sites: SphI PstI SalI BamHI SmaI KpnI HpaI_
Polylinker sites: SphI PstI SalI BamHI SmaI KpnI HpaI
Other unique sites: ScaI SspI
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
replicon: ars1
replicon: pMB1
marker(s): LEU2
operator: tet, <-
promoter: CaMV, <-
marker(s): ampR, <-
Vector: pSLF101 (phagemid)
Promoters: Promoter CaMV
Construct size (kb): 6.800000190734863
Features: marker(s): LEU2
marker(s): ampR
operator: tet
promoter: CaMV
replicon: ars1
replicon: pMB1
expression vector
Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.2; BamHI--6.8; EcoRI--3.9, 1.2.
The vector contains the constitutive CaMV promoter adjacent to the tet operator.
The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.
A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

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