pSLF101 (ATCC® 87619)

Applications: expression vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pSLF101
Depositors SL Forsburg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.8000001907348630
Intact vector size: 6.800
Type of vector: phagemid
Cloning sites: SphI PstI SalI BamHI SmaI KpnI HpaI_
Polylinker sites: SphI PstI SalI BamHI SmaI KpnI HpaI
Other unique sites: ScaI SspI
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
replicon: ars1
replicon: pMB1
marker(s): LEU2
operator: tet, <-
promoter: CaMV, <-
marker(s): ampR, <-
Vector: pSLF101 (phagemid)
Promoters: Promoter CaMV
Construct size (kb): 6.800000190734863
Features: marker(s): LEU2
marker(s): ampR
operator: tet
promoter: CaMV
replicon: ars1
replicon: pMB1
expression vector
Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.2; BamHI--6.8; EcoRI--3.9, 1.2.
The vector contains the constitutive CaMV promoter adjacent to the tet operator.
The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.
A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

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