REP4X (ATCC® 87604)

Applications: expression vector  /  Depositors: SL Forsburg

Designations REP4X
Permits and Restrictions

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Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 8.5000000000000000
Vector: REP4X (phagemid)
Promoters: Promoter for expression nmt1 (full strength)
Construction: pUC119
Construct size (kb): 8.5
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt1 (full strength)
replicon: ars1
replicon: pMB1, f1
MCS: XhoI...SmaI
expression vector
Restriction digests of the clone give the following sizes (kb): XhoI--8.5; HindIII--5.5, 1.9, 1.1; EcoRI--7.1, 1.4.
REP3X (ATCC 87603) and REP4X (ATCC 87604) differ in that REP3X contains LEU2 (2.2 kb) and REP4X contains ura4+ (1.8 kb).
REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)
due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).
REP4X was constructed by deleting the ATG in the polylinker of the original REP4 plasmid. An XhoI linker was cloned into the blunted BalI-SalI cut REP4 plasmid. The SalI site is preserved.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Susan L Forsburg, personal communication

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component of:ATCC 87685
Shipped frozen
Shipping Information

 Freeze dried E. coli containing the phagemid