YEplac195 (ATCC® 87589)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Designations YEplac195
Permits and Restrictions

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Depositors RD Gietz
Biosafety Level 1
Vector Information
Size (kb): 5.241
Vector: YEplac195 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Construct size (kb): 5.241
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
This is a YE-type (yeast episomal) vector with the URA3 marker.
The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
Restriction digests of the clone give the following sizes (kb): AvaI--4.2, 0.7; EcoRV--5.0; PstI--5.0.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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Shipped frozen
Shipping Information

 Freeze dried E. coli containing the plasmid