YCplac22 (ATCC® 87585)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

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Designations YCplac22
Depositors RD Gietz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.8540000915527340
Intact vector size: 4.854
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
Other unique sites: EcoRV XcaI AatII NarI SpeI NaeI NheI BglII
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): TRP1, <-, 2063-2740
marker(s): ampR, ->, 3053-3913
replicon: pMB1, 4666-4666
Vector: YCplac22 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Construct size (kb): 4.854000091552734
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.9; HindIII--4.8; XbaI--4.8.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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