Size (kb): 10.1999998092651400
Vector: pLGdelta312 (plasmid)
Promoters: Promoter CYC1 promoter (including the CYC1 UAS)
Construct size (kb): 10.19999980926514
Features: marker(s): URA3
promoter: CYC1 promoter (including the CYC1 UAS)
replicon: 2 micron ori
coding sequence: lacZ
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): SmaI--10.3; HindIII--8.0, 2.1; EcoRI--4.5, 3.15, 2.25.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511
, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
The vector was constructed by Lenny Guarente.
Guarente L, Hoar E. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA" box. Proc. Natl. Acad. Sci. USA 81: 7860-7864, 1984. PubMed: 6096863