pRS422 (ATCC® 87479)

Clone Type: Vector YE-type (episomal) shuttle vector  /  Depositors: JD Boeke

Designations pRS422
GenBank Number


Depositors JD Boeke
vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
Intact vector size: 6.868
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS421
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, →, 575-2290
replicon: f1, ←, 2495-2951
promoter for in vitro transcription: T7, →, 3121-3140
MCS: KpnI...SacI, →, 3148-3250
promoter for in vitro transcription: T3, ←, 3267-3286
coding sequence: lacZ', ←, 2952-3311
promoter: lac, ←, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, ←, 4468-5328
replicon: 2 micron, 5460-6801
Vector: pRS422 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS421
Marker(s): ampR,ADE2
Construct size (kb): 6.868
Features: marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
coding sequence: lacZ'
Target Gene: , phosphoribosylaminoimidazole succinocarboxamide synthetase
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Component of: ATCC 87480
Component of: ATCC 87530
Component of: ATCC 87535
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--4.6, 2.2; EcoRI--6.8.
ade2 phenotype produces red colonies when grown on adenine containing media.
This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation.

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801