Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Distribution host: Escherichia coli
HB101 (ATCC 33694)
Size (kb): 6.5339999198913570
DESCRIPTION OF VECTOR:
Intact vector size: 6.534
Type of vector: plasmid
Cloning sites: SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI
Other unique sites: SacI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: SalI...XbaI, ->, 3394-3452
promoter for expression: CYC1, <-, 3452-3739
marker(s): ampR, <-, 4986-5916
replicon: CEN6/ARSH4, ->, 6000-6514
Vector: p415 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construct size (kb): 6.533999919891357
Features: marker(s): LEU2
promoter for expression: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.2, 0.3; KpnI--5.0, 1.6; EcoRI--4.3, 2.3.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504