Escherichia coli 1046 (pCRE1) (ATCC® 77368)

Clone Type: Clone  /  Depositors: IN Maruyama

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Designations Escherichia coli 1046 (pCRE1)
Depositors IN Maruyama
Applications
Produces protein recombinase, site-specific
Vector
Construct size (kb): 5.960
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: ScaI
Vector end: ScaI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: PvuII
Insert end: PvuII
Insert size (kb): 1.6
Cross references:
Gene product: recombinase, site-specific [cre]
Target Gene: recombinase, site-specific
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Related Products
Component of: ATCC 77369
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Freeze-dried E. coli 1046
Comments This E. coli contains the pCRE1 plasmid and expresses the Cre recombinase from a lacZ promoter.  Insert-containing lambdaMGU2 (77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR surviviors of phage infection.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35.
References

Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972