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Escherichia coli (Migula) Castellani and Chalmers (ATCC® 700926)

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Strain Designations: MG1655  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations MG1655
Derived from parent strain W1485 by acridine orange curing of the F plasmid
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype OR:H48:K-
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- lambda- ilvG- rfb-50 rph-1
Genome Sequenced Strain

Escherichia coli str. K-12 substr. MG1655, complete genome

Type Strain no
Antigenic Properties Serotype OR:H48:K-

University of Wisconsin Genome project strain

Component of MSA-1000, MSA-1001, MSA-1002, MSA-1003, MSA-1006, MSA-2002, MSA-2003, MSA-2006, MSA-3000, MSA-3001, and MSA-3002.

Medium ATCC® Medium 2511: M9 Minimal Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- lambda- ilvG- rfb-50 rph-1
Name of Depositor G Plunkett
Special Collection NCRR Contract
Chain of Custody
ATCC <-- G Plunkett <-- M Berlyn <-- M Guyer
Derived from parent strain W1485 by acridine orange curing of the F plasmid

Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503

Guy Plunkett, personal communication

Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388

Cross References

Nucleotide (GenBank) : U00096 Escherichia coli str. K-12 substr. MG1655, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019