Enterococcus dispar Collins et al.

1. Open vial according to enclosed instructions.
2. From a single tube of #44 broth (5 to 6 mL), withdraw approximately 0.5 to 1 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate all tubes and plate at 30°C for 24 hours.

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