Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47059)

Strain Designations: BNN132  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations BNN132
Application
transformation host
Type Strain no
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information Distributed: freeze-dried
Preceptrol® no
Genotype endA1 gyr96 hsdR17 supE44 relA1 delta(lac-proAB) (F' traD36 proA+ proB+ lacIq delta(lacZ)M15) lambdaKC(kan-cre)
Type Strain no
Comments
Phenotype verified: supE44, kanamycin resistance, and lac-.
Host for generating plasmid derivatives from recombinants in bacteriophage lambda vectors that contain lox sites flanking the plasmid portion. Example: lambdaYES.
JM107 lysogenic for lambdaKC, a bacteriophage lambda derivative containing the cre gene for the site-specific recombinase from bacteriophage P1 that recognizes lox sites. The phage also contains neo from Tn5, conferring kanamycin resistance.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype endA1 gyr96 hsdR17 supE44 relA1 delta(lac-proAB) (F' traD36 proA+ proB+ lacIq delta(lacZ)M15) lambdaKC(kan-cre)
Name of Depositor SJ Elledge
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation