Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47025)

Strain Designations: BNN102 [C600hflA150, Y1073]  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations BNN102 [C600hflA150, Y1073]
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Genotype F- hflA150::Tn10 supE44 lacY1 thr-1 leuB6 mcrA1 mcrB thi-1 fhuA21 e14- hsdR lambda-
Type Strain no
Comments
Positive selection host for recombinant lambdagt10 libraries. By suppressing plaque formation of cI+ phage, it plates only recombinant phage.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- hflA150::Tn10 supE44 lacY1 thr-1 leuB6 mcrA1 mcrB thi-1 fhuA21 e14- hsdR lambda-
Name of Depositor R Young, RW Davis
References

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985