AM6 [EC-AM6, pAM6] (ATCC® 40101)

Organism: Hepatitis B virus, Hepatitis B virus  /  Clone Type: Clone  /  Depositors: Scripps Clinic and Research Foundation

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Designations AM6 [EC-AM6, pAM6]
Species Hepatitis B virus, Hepatitis B virus
Depositors Scripps Clinic and Research Foundation
Construct size (kb): 7.5
DNA: genomic
Insert lengths(kb): 3.200000047683716
Insert Size (kb) 3.200
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--4.5, 1.85, 1.35; HindIII--7.5; EcoRI--7.5; BglII/PstI--5.2, 1.35, 0.96.
A BamHI digest cuts the insert into fragments of 1.85 kb and 1.35 kb. The 1.35 kb fragment contains coding sequences for HBsAg and most of region P. The 1.85 kb fragment encodes HBcAg and most of the X gene product.
AM6 is available as ATCC 39630, 40101, 45020 and 45020D.
Molecular clone of linearized hepatitis genome
The 1350 bp BamHI fragment encodes both group and subtype-specific determinants of HBsAg.
The virus was isolated from plasma (subtype adw) from an HBsAg-positive donor. DNA was extracted from purified Dane particles.
The complete viral genome was linearized with BamHI and inserted into pBR322.
Classification Hepadnaviridae, Orthohepadnavirus

Moriarty AM, et al. Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector. Proc. Natl. Acad. Sci. USA 78: 2606-2610, 1981. PubMed: 6264484

Moriarty AM. SV40 expression vector containing HBxAg as an expression marker. US Patent 4,942,125 dated Jul 17 1990