pGCAT-A (ATCC® 37640)

Applications: expression vectorpromoter-cloning vectorshuttle vectorvector permitting RNA synthesis in vitro  /  Depositors: T Frebourg, O Brison

Designations pGCAT-A
Depositors T Frebourg, O Brison
Biosafety Level 1
Vector Information
Size (kb): 4.5500001907348630
Vector: pGCAT-A (plasmid)
Promoters: Promoter T7
Construction: pSB1, pGEM4
Construct size (kb): 4.550000190734863
Features: insert detection: CAT
marker(s): ampR
promoter: SP6, T7
replicon: pMB1
expression vector
promoter-cloning vector
shuttle vector
vector permitting RNA synthesis in vitro
Restriction digests of the clone give the following sizes (kb): PvuII--4.3, 0.2; HincII--3.0, 1.5; EcoRI--4.5.
A plasmid shuttle vector for investigating promoter and/or enhancer elements.
The sequence at the junction of the multiple cloning site and CAT is GAATTAGCTT and at the junction of SV40 and SP6 is GGATCAATTC.
The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641).
The BamHI/HindIII fragment of pSB1 containing CAT was cloned into the EcoRI site of pGEM-4 (all ends blunt). Contains the SV40 polyadenylation site and donor and acceptor sites of SV40 small t antigen intron.
Has a polylinker upstream of the reporter CAT gene and demonstrates low basal CAT expression in eukaryotic cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Frebourg T, Brison O. Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells. Gene 65: 315-318, 1988. PubMed: 2842234

Thierry Frebourg, personal communication

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