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pMMB67EH (ATCC® 37622)

Applications: expression vectorshuttle vector  /  Depositors: E Lanka

Designations pMMB67EH
Permits and Restrictions

View Permits

Depositors E Lanka
Biosafety Level 1
Vector Information
Size (kb): 8.83
Vector: pMMB67EH (plasmid)
Promoters: Promoter tac
Construction: pMMB66EH (ATCC 37620), M13mp18 polylinker
Marker(s):ampR
Construct size (kb): 8.83
Features: marker(s): ampR
promoter: tac
replicon: RSF1010 (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Contains the following sites separated by (bp): EcoRI-785 -AhaIII-97 -ScaI-112 -PvuI-2101 -AccI-1848 -ScaI-55 -AccI-2039 -BstEII-372 -PvuII-93 -PvuII-94 -HpaI-320 -BstEII-182 -MluI-732 -EcoRI.
Restriction digests of the clone give the following sizes (kb): AccI--3.7, 3.1, 1.8; BamHI--8.8; HindIII/PvuII--7.2, 1.3. NOTE: A CONSTANT 3.0 KB BAND MAY BE OBSERVED. IT IS NOT A CONTAMINANT BUT APPEARS TO BE AN ANOMALY.
In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter.
An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
The M13mp8 polylinker of pMMB66EH (ATCC 37620) was replaced with the M13mp18 polylinker.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457

Jon A Kornacki, personal communication

Erich Lanka, personal communication

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid.