pAV1 (ATCC® 37215)

Organism: Adeno-associated virus 2  /  Clone Type: Clone  /  Depositors: CA Laughlin

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Designations pAV1
Species Adeno-associated virus 2
Depositors CA Laughlin
Contains the complete viral genome for use in site-specific mutagenesis.
Construct size (kb): 9.0
DNA: genomic
Insert lengths(kb): 4.7
Gene product: human adeno-associated virus type 2
Insert Size (kb) 4.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): BglII--4.7, 4.0; HindIII--6.6, 1.9; EcoRI--3.9, 2.8, 1.9.
pAV1 (ATCC 37215) and pAV2 (ATCC 37216) both contain the complete AAV2 genome, but in opposite orientations. The pAV2 vector has fewer polylinker sites than that of pAV1 (HindIII, ClaI, and one EcoRI site have been deleted).
Contains the complete viral genome for use in site-specific mutagenesis. Transfection into human 293 cells with an Ad helper virus results in infectious AAV virus particles.
Classification Parvoviridae, Dependovirus, Adeno-associated virus-2

Laughlin CA, et al. Cloning of infectious adeno-associated virus genomes in bacterial plasmids. Gene 23: 65-73, 1983. PubMed: 6352411